Composite

Part:BBa_J119126:Design

Designed by: Caleb J. Carr   Group: Eckdahl_Lab   (2012-09-28)


P5-Bd24 ligated to trpR (S05028+J119124)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 305


Design Notes

The scar that would result, as it ends up just between the cDog and the gene to be translated.


Source

The S05028 part was synthesized by the Davidson team, and the J119124 part was cloned out of the native genome of E. coli using primers.

References